A High-Performance Thin Layer Chromatography Densitometric Method for the assay of Mebendazole Tablets

Vicky Manyanga; Emmanuel Kimaro; Joseph Sempompe; Eliangiringa Kaale; Mhina Chambuso

Vicky Manyanga 1Department of Medicinal Chemistry, Muhimbili University of Health and Allied Sciences, P.O. Box 65013, Dar Es Salaam, Tanzania
Emmanuel Kimaro 1Department of Medicinal Chemistry, Muhimbili University of Health and Allied Sciences, P.O. Box 65013, Dar Es Salaam, Tanzania
Joseph Sempompe 1Department of Medicinal Chemistry, Muhimbili University of Health and Allied Sciences, P.O. Box 65013, Dar Es Salaam, Tanzania
Eliangiringa Kaale 2Pharm R&D Laboratory, School of Pharmacy, Muhimbili University of Health and Allied Sciences P.O. Box 65013, Dar Es Salaam, Tanzania.
Mhina Chambuso Department of Medicinal Chemistry, Muhimbili University of Health and Allied Sciences, P.O. Box 65013, Dar Es Salaam, Tanzania.

Keywords: HPTLC, method validation, assay, mebendazole

Abstract

A simple, precise and accurate high-performance thin layer chromatographic (HPTLC) method was developed for the assay of mebendazole tablets. The separation was carried out by using HPTLC Silica gel 60 F254, (20 × 10 cm) with 250 μm thickness using ethyl acetate - ammonia 25% solution (25:0.5) as a mobile phase. HPTLC separation of the drug was followed by densitometry measurement at 310 nm. Mebendazole was satisfactorily resolved with retention factor (Rf) values of 0.51 ± 0.02. The method complied with International Conference on Harmonization acceptance criteria for linearity (250 - 600 ng), precision, accuracy and specificity.