A High Performance Liquid Chromatography Method for the Determination of Artemisinin in Artemisia annua L. Leaf Extracts

  • Sarah Mwangi National Quality Control Laboratory, Nairobi, Kenya
  • Kennedy Abuga Department of Pharmaceutical Chemistry, University of Nairobi
  • Nelly Mungai Department of Pharmacology and Pharmacognosy, University of Nairobi
  • Julius Mwangi Department of Pharmacology and Pharmacognosy, University of Nairobi
Keywords: Artemisia annua, artemisinin content, crude extract

Abstract

A simple, sensitive, accurate and precise high performance liquid chromatography (HPLC) method for determination of artemisinin in crude plant material was developed and validated. Optimal separation of artemisinin from matrix components in the plant extracts was achieved using a Waters XTerra® RP18 , 5 mm, 250 × 4.6 mm column, maintained at 40 °C, a mobile phase consisting of 0.05 M potassium phosphate buffer, pH 6.0 - acetonitrile (60:40) containing 5 mM hexane sulfonate in isocratic flow. The mobile phase flow rate was 1.0 ml/min while elution was monitored at 216 nm. The method satisfied the International Conference on Harmonization (ICH) validation criteria for linearity, accuracy, precision and sensitivity. The developed method is applicable in routine quality control of Artemisia annua crude extracts.

How to Cite
Mwangi, S., Abuga, K., Mungai, N., & Mwangi, J. (1). A High Performance Liquid Chromatography Method for the Determination of Artemisinin in Artemisia annua L. Leaf Extracts. The East and Central African Journal of Pharmaceutical Sciences, 23(2), 48-53. Retrieved from https://uonjournals.uonbi.ac.ke/ojs/index.php/ecajps/article/view/580
Issue
Vol 23 No 2 (2020): May-August 2020
Section
Articles